Accurate flow control for cancer research

In basic cancer research, a main aim is to unravel differences between normal cells and cancer cells in order to exploit these differences in the hunt for specific cancer vulnerabilities, so that ways can be found to attack cancer cells while leaving normal cells unaffected. 

Since living cells reveal much more information than dead ones, at the Netherlands Cancer Institute (NKI) advanced microscopy techniques are used to extract information about proteins and their interactions in single living cancer cells. To investigate these living cells in their ‘natural habitat’, Bronkhorst was asked to deliver devices for supplying water vapour and gases such as nitrogen, oxygen and carbon dioxide to mimic the conditions in our body.

Process solution

Bronkhorst delivered three EL-FLOW Select mass flow controllers with read-out units for the controlled supply of air, nitrogen and carbon dioxide gases. By adjusting the relative flows of these gases, an atmosphere was created that contained 2-19 % O2, 0-20 % CO2 and 80-100% N2. This range of gases was used in the incubator to investigate the behaviour of cancer cells, for example to investigate how cancer cells migrate during metastasis or to explore how cells use chemical signals to communicate with each other. Investigations at low oxygen content are especially relevant, as solid tumours are well known to be hypoxic - i.e. they contain less than a few % of oxygen - and this completely alters the physiology of the cells, as well as their response to anti-cancer drugs. All experiments conducted under these controlled conditions showed consistent results.