Application requirements
A dedicated incubator needs to be established that houses the living cells and that ‘keeps them alive’ under conditions that resemble the various oxygen tensions and other conditions encountered in the human body, while being able to be observed by the microscope.
To this end, the moisturised atmosphere in the incubator needs to be controlled accurately at a temperature of 37 °C, should contain at least 5% CO2, and the oxygen content must be adjusted between 2% and 20%.